Spectrophotometric Determination of Tetracycline, in pharmaceutical formulations by oxidative coupling

Abstract

This research includes the development of spectrophotometric method for determination of Tetracycline via oxidative coupling with the N,N-Diethyl-p-phenylene diamine reagent in presence of oxidizing agentN-Bromosuccinimide in the alkaline medium oxidation time 15 min to form a colored-dye violet, which dissolved in water and showed the highest intensity of absorption at λmax 552nm, the method has been obeyed Beer's Law in the concentration range (10-32μg. ml-1), with molar absorptivity of 10579 L. mol-1. Cm-1, Sandel Index value 0.0454 μg. cm-2, the detection limited 0.329 μg. ml-1, with correlation coefficient 0.9942 , the relative standard deviation of the method does not exceed 0.302% the proposed method was applied successfully for the determination of Tetracycline Hydrochloride on pharmaceutical preparation by both the direct and standard addition methods for three concentrations, the recovery ranged between 98.8-102.71%. the evaluations of results of this method was a achieved applying t and F tests. The results showed that there is no significant difference between the proposed and standard method, and thus the proposed method is of good validity for the determination Tetracycline in its pharmaceutical preparations