Abstract
A simple and sensitive spectrophotometric method has been developed for the determination of some phenothiazine derivatives as pure and in dosage form. The method is based on oxidation of phenothiazine s by N-chlorosucci nimide in hydrochloric acid medium to give red coloured species having maximum absorptions in the range 516.5534.5nm. Beer‘s law is obeyed over the concentration ranges 2-28, 2-32 and 4-40µg.ml -1 with apparent molar absorptivity of 6.16 ×10³, 5.89 ×10³ and 5.34×10³ l.mol -1 .cm -1 for phenothiazine, promethazine hydrochloride and chlorpromazine hydrochloride respectively. Additives and excipients normally found in dosage forms do not interfere.
Highlights
Phenothiazines which were introduced in the1950’s as antipsychotic drugs are still widely used in the treatment of moderate to severe mental illnesses including schizophrenia
The present work reports the use of N-chlorosuccinimide as oxidant reagent for the spectrophotometric determination of some phenothiazine derivatives
Recommended procedure In a series of volumetric flasks of 25-ml, aliquots of standard solutions of phenothiazine, promethiazine hydrochloride and chlorpromazine hydrochloride with concentrations of 228, 2-32 and 4-40 μg ml-1, respectively in final volume were added separately, followed by addition of 4 ml N-chlorosuccinimide and 2 ml of hydrochloric acid, the contents were diluted to the mark with distilled water and mixed thoroughly, the solutions were left for 15 minutes at room temperature and the absorbance was measured at 516.5, 517 and 534.5 nm for the above drugs, respectively against their respective reagent blank and a calibration graph was constructed
Summary
Phenothiazines which were introduced in the1950’s as antipsychotic drugs are still widely used in the treatment of moderate to severe mental illnesses including schizophrenia. Many direct spectrophotometric methods for the determination of phenothiazines have been suggested based on the oxidation of the drugs to the radical cations and subsequent measurement of absorbance, with the use of such oxidizing agents as molybdate (Basavaiah and Krishnamurthy, 1998), chloramine-T (Basavaiah and Swamy, 2001 ; Revanasiddappa and Veena, 2008), hexacyanoferrate (III) and Ferriin (Basavaiah and Swamy,2002), vanadate (Misiuik and Tarasiewicz, 1993), N-bromosuccinimide (Taha et al, 1983), cerium(IV) (Misiuik and Tarasiewicz,1996), 2-iodobenzoate (Hassan et al.,1989) and p-benzoquinone (Murthy and Seetharamappa, 2000). The present work reports the use of N-chlorosuccinimide as oxidant reagent for the spectrophotometric determination of some phenothiazine derivatives
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