Spectrophotometric Determination of Raloxifene Hydrochloride in Pharmaceutical Formulations

M Mathrusri Annapurna,B V V Ravi Kumar,M E Bhanoji Rao

doi:10.1155/2007/480625

M Mathrusri Annapurna, B V V Ravi Kumar + Show 1 more

Open Access

https://doi.org/10.1155/2007/480625

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Abstract

Three new simple, sensitive, rapid and economical spectrophotometric Methods (A, B and C) have been developed for the determination of Raloxifene Hydrochloride in pharmaceutical bulk and tablet dosage form. Method A is based on the formation of yellow colored chromogen with 0.1N Sodium hydroxide exhibiting maximum absorbance against the corresponding reagent blank. The method B is based on the reaction of Raloxifene with Ferric chloride and 1, 10-phenanthroline to form blood red colored chromogen. The method C is based on the formation of blood red colored chromogen with Ferric chloride and 2, 2' bipyridyl. The absorbencies of the chromogens were measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

Highlights

Raloxifene Hydrochloride[1] is a new anti-osteoporotic agent, with a chemical name 6-Hydroxy-2(4-hydroxy phenyl) benzo {b} thien-3-yl] [4-{2-(1-piperidinyl)-ethoxy}-phenyl] methanone
The reagents used in Method B were Ferric chloride (0.03M) and 1, 10-phenanthroline (0.01M) and in Method C were Ferric chloride (0.03M) and 2, 2' bipyridyl (0.01M)
Aliquots of standard solutions containing 0.5-1.5 ml of 100 μg/ml and 1 mg/ml were transferred into a series of 10ml graduated test tubes separately and the total volume was made up to 10 ml with 0.1M sodium hydroxide

Summary

Introduction

Raloxifene Hydrochloride[1] is a new anti-osteoporotic agent, with a chemical name 6-Hydroxy-2(4-hydroxy phenyl) benzo {b} thien-3-yl] [4-{2-(1-piperidinyl)-ethoxy}-phenyl] methanone. It is effective in the treatment of breast cancer[2, 3] and in reduction of fracture risk[4]. It is an estrogenagonist in bone, where it exerts an anti-resorptive effect. The authors have developed three simple and sensitive spectrophotometric methods for the determination of Raloxifene hydrochloride. The reagents used in Method B were Ferric chloride (0.03M) and 1, 10-phenanthroline (0.01M) and in Method C were Ferric chloride (0.03M) and 2, 2' bipyridyl (0.01M)

Method A

Method B

Method C

Results and Discussion