Abstract
A simple spectrophotoemtric method for the determination of mesalazine [5-aminosalicylic acid; (5-ASA)] in aqueous solution is achieved. The method is based on the reaction of mesalazine, with excess nitrite, in an acidic medium, to produce the corresponding diazonium salt. After the removal of residual nitrite with sulphamic acid, the diazonium salt is coupled with resorcinol reagent in basic medium to produce, an intense orange coloured water-soluble and stable azo-dye which exhibits maximum absorption at 471nm. Beer’s law is obeyed in the concentration range of 10-300µg of mesalazine in a final volume of 25 ml i.e., 0.4-12 ppm with a molar absorptivity of 2.9480×104 l.mol-1.cm-1 and Sandell sensitivity index of 0.0051µg.cm-2, a relative error of -0.96 to -0.23% and relative standard deviation of ±1.05 to ±0.37% depending on the concentration level. The proposed method has been applied successfully to determine mesalazine in pharmaceutical preparation (capsules).
Highlights
Mesalazine [5-Amino salicylic acid; (5-ASA)] is an agent widely used in the treatment of inflammatory bowel disease (IBDs), is metabolized in organism to the principal biotransformation product, N-acetyl-5-ASA, is a polar compound and besides it exhibits amphoteric properties (Liu et al, 1995 and Nobilis et al, 2006)
Different methods have been reported for the determination mesalazine including: liquid chromatographic technique which was used to determine 5-ASA and its metabolite N-acetyl-5-aminosalicylic acid in plasma and urine by using a spectrofluoimetric detector, excitation at 311nm
Mesalazine was determined by three different methods: the first method (HPLC) was carried out with a C18 column and a mobile phase was constituted of 30 mmol/l monobasic phosphate buffer and methanol (70:30; v/v), with 25% tetrabutylammonium hydrogen sulphate used ultraviolet detection at 254 nm, the second method
Summary
Mesalazine [5-Amino salicylic acid; (5-ASA)] is an agent widely used in the treatment of inflammatory bowel disease (IBDs), is metabolized in organism to the principal biotransformation product, N-acetyl-5-ASA, is a polar compound and besides it exhibits amphoteric properties (Liu et al, 1995 and Nobilis et al, 2006). A spectrophotometric method for the determination of microgram amounts (0.168μg/ml) of mesalazine based on the oxidative coupling with 2,6-xylenol in the presence of sodium metaperiodate in alkaline medium to form a blue indophenol dye which has maximum absorption at 610 nm with a molar absorptivity is 13316l.mol-1.cm-1 (AlFakhry, 2006). Based on the diazotization of 5-ASA and coupling with resorcinol reagent and applying the method to the determination of 5-ASA in pharmaceutical preparation (capsules).
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