Abstract
A simple and sensitive spectrophotometric method is proposed for the determination of mesalazine (MZ) in some of its pharmaceutical preparations. The method is based on the diazotization of mesalazine by reaction with sodium nitrite in an acidic medium and then coupling of diazonium salt corresponding to mesalazine with 2,6-dihydroxytoluene reagent in basic medium to produce an intense orange colored water-soluble and stable azo-dye for at lest two hrs, and exhibits maximum absorption at 453 nm. Beer’s law is obeyed in the concentration range of 2.5-100 µg of mesalazine in a final volume of 10 ml i.e.,0.25-10.0 ppm with a molar absorptivity of 4.6×104 l. mol-1. cm-1 and sandell sensitivity index of 0.00332 µg. cm-2 . A relative error is -0.48 to -4.80 and relative standard deviation of ±0.01 to ±0.17 depending on the concentration level. The proposed method has been applied successfully to determine mesalazine in pharmaceutical preparations, tablets and suppositories. .
Highlights
Mesalazine (MZ), known as mesalamine or 5-aminosalicylic acid (5-ASA), is an anti-inflammatory drug used to treat inflammation of the digestive tract (Crohn’s disease) and mild to moderate ulcerative colitis (Kanala et al, 2013)
Several methods have been reported for determination of mesalazine in pure form or in pharmaceutical preparations
During the investigation, 25 μg of mesalazine was taken and the volumes are brought to 10 ml with distilled water
Summary
Mesalazine (MZ), known as mesalamine or 5-aminosalicylic acid (5-ASA), is an anti-inflammatory drug used to treat inflammation of the digestive tract (Crohn’s disease) and mild to moderate ulcerative colitis (Kanala et al, 2013). الملخص تعتمد الطريقة.يتضمن البحث اقتراح طريقة طيفية بسيطة وحساسة لتقدير الميزالازين في بعض مستحضراته الصيدلانية على أزوتة الميزالازين وذلك بتفاعله مع نتريت الصوديوم في الوسط الحامضي يتبعه اقتران ملح الدايازونيوم المقابل – ثنائي هيدروكسي تولوين في الوسط القاعدي لتكوين صبغة ازوية برتقالية اللون ذائبة في الماء6،2 للميزالازين مع الكاشف انطبق قانون بير في مدى. نانوميتر453ومستقرة لمدة ساعتين على الأقل وتعطي اعلى امتصاص عند الطول الموجي وكانت قيمة،( جزء لكل مليون10 -0.25) مللتر10 مايكروغرام من الميزالازين في حجم نهائي100 -2.5 التراكيز والخطأ،2-سم. مايكروغرام0.00332 وقيمة دلالة ساندل،1-سم. Several methods have been reported for determination of mesalazine in pure form or in pharmaceutical preparations.
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