Abstract
Optimal working conditions for the spectrophotometric determination of magnesium in biological fluids have been studied. It has been found that 0.7 – 1.4 × 10 −4 m reagent concentrations should be applied when 20–50 μl of specimen to 4.0 ml final mixed Xylidyl Blue II buffer solution is used. It has been further observed that calcium, sodium, potassium, and chlorides interfere seriously with the magnesium determination in giving false higher results. This interfering effect may, however, be corrected by adding all these ions in approximately physiological concentrations to the standard magnesium solution, which should be put up with each set of determinations. By this modification, the Xylidyl Blue II method gives in analyses of biological fluids results concordant with those obtained by atomic absorption spectrophotometry, as has been shown on magnesium determination in 48 serum and 16 cerebrospinal fluid specimens.
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