Spectrophotometric Determination of Fluroral and Uranine in Milk

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Summary To render antibiotic-containing milk easily recognizable, it has been suggested that a dye marker be added to veterinary antibiotic preparations which are to be infused into the udder. Ideally, the dye would be eliminated from the udder at the same rate as the antibiotic, and the milk should be discarded as long as a trace of dye was detected. A mixture of two fluorescein dyes, (Fluroral and Uranine), has shown promise for this purpose. To properly evaluate such a procedure, quantitative methods for measuring the marker content of milk were needed. Therefore, simple and rapid spectrophotometric methods have been devised for the quantitative determination of fat-soluble fluorescein (fluroral) and water-soluble fluorescein (uranine) in milk. Reproducibility values ranged from 92 to 100%, and recovery values from 94 to 103%.