Abstract
Analytical application of 2-acetylpyridine thiosemicarbazone (APT) and 2-acetylpyridine semicarbazone is described for the direct non-extractive spectrophotometric determination of cobalt. The reagents react with cobalt in acidic medium (pH 6.0, sodium acetate-acetic acid buffer) to form orange yellow colored 1: 2 (M: L) complexes. The colour reactions are instantaneous and absorbance values remain constant for over 24 h. The molar absorptivity and Sandell's sensitivity of APT and APS methods are found to be 1.25 x 10 4 and 1.45 x 10 4 L mol -1 cm -1 and 0.0047 and 0.004 μg cm -2 of Co II respectively. The systems obey Beer's law in the range of 0.236-2.357 μg/ml of Co II . Since APS method is more sensitive it was applied for the determination of cobalt in biological samples.
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