Spectrophotometric Determination of Ceftibuten in Bulk and in Pharmaceutical Formulations

R Vijayalakshmi,P Srujana Kumari,P Sruthi,P Bhargavi,M D Dhanaraju

doi:10.1155/2010/120417

Abstract

A simple and cost effective spectrophotometric method is described for the determination of ceftibuten in pure form and in pharmaceutical formulations. The method is based on the formation of blue colored chromogen, when the drug reacts with Folin-Ciocalteu (F-C) reagent in alkaline medium. The colored species has an absorption maximum at 798 nm and obeys Beer’s law in the concentration range 10-50 mcg/mL. The apparent molar absorptivity and sandell’s sensitivity were 0.4583x104and 0.3x104respectively. The slope and intercept of the equation of the regression line are 4.5x10-3and 1.3x10-5respectively. The optimum experimental parameters for the reaction have been studied and the validity of the described procedure was assessed. Statistical analysis of the results has been carried out revealing high accuracy and good precision. The proposed method was successfully applied for the determination of ceftibuten in pharmaceutical formulations.

Highlights

Ceftibuten1(CTB) is chemically [6R-6α-7β(R*)]-7-[α-amino-4-hydroxy phenyl)-acetyl]amino]-8oxo-3-(1-propenyl)-5-thia-1-azabicyclo[4.2.o]oct-2-ene-2-carboxylic acid monohydrate
Literature survey reveals that the drug was determined by using HPLC3-5 and some spectrophotometric methods6
According to literature survey there is no method reported for ceftibuten by visible spectrophotometry

Summary

Introduction

Ceftibuten1(CTB) is chemically [6R-6α-7β(R*)]-7-[α-amino-4-hydroxy phenyl)-acetyl]amino]-8oxo-3-(1-propenyl)-5-thia-1-azabicyclo[4.2.o]oct-2-ene-2-carboxylic acid monohydrate. There was an attempt made to develop a simple and sensitive spectophotometric method for the estimation of ceftibuten in pure drug and in pharmaceutical formulations. The method uses the well known reduction reaction involving Folin-Ciocalteu (F-C) reagent and ceftibuten resulting in the formation of a blue chromogen that could be measured at 798 nm.

Results

Conclusion