Spectrophotometric Determination of Cefixime Following Simple Diazotization and Coupling with ?-Naphthol

Samar A Darweesh

doi:10.31351/vol26iss2pp1-6

Abstract

Cefixime (CFX) was treated with sodium nitrite and hydrochloric acid for diazotization reaction followed by coupling with ?-Naphthol in alkaline medium to form, a yellow colored azo dye compound which exhibits maximum absorption (?max) at 412 nm where the concentration of (CFX) was determined spectrophotometrically. The optimum reaction conditions and other analytical parameters were evaluated. Beer’s law was obeyed in the concentration range of (1-20) ?g.mL-1 with a molar absorptivity of 34870.5 L.mol-1.cm-1. The limit of detection was found to be 0.1090 ?g.mL-1 and the Sandell's sensitivity value was 0.0130 ?g.cm-2. The proposed method could be successfully applied to the determination of (CFX) in pharmaceutical formulations.

Highlights

Cefixime is a semisynthetic, cephalosporin antibacterial for oral administration (Scheme 1).Scheme [1]: The chemical structure of cefixime.Chemically it is (6R,7R)-7-{(2-(2-Amino-1,3thiazol-4-yl)-2-(Carboxy-methoxyimino )acetyl ) amino } – 3 – ethenyl – 8 – oxo – 5 – thia – 1 azabicyclo(4 . 2 . 0 ) oct – 2 – ene – 2 - carboxylic acid trihydrate
It Acts by inhibiting an enzyme transpeptidase, involved in the building of bacterial cell walls. It is used in lower respiratory tract infections. It is helpful in acute urinary tract infections, biliary tract infections, sinusitis, acute otitis media, peptic ulcer and many more [1]. cefixime has been determined by the development of several analytical techniques such as flow injection analysis [2], HPLC [3,4,5], potentiometric method [6], voltametric method [7], spectrophotometric method [8,9,10,11,12]
The present study describes the use of α-naphthol as a chromogenic reagent in the development of simple, sensitive and a rapid spectrophotometric method for the estimation of CFX with reasonable precision, accuracy

Summary

Introduction

Cephalosporin antibacterial for oral administration (Scheme 1). Scheme [1]: The chemical structure of cefixime It is (6R,7R)-7-{(2-(2-Amino-1,3thiazol-4-yl)-2-(Carboxy-methoxyimino )acetyl ) amino } – 3 – ethenyl – 8 – oxo – 5 – thia – 1 azabicyclo(4 . It Acts by inhibiting an enzyme transpeptidase, involved in the building of bacterial cell walls It is used in lower respiratory tract infections. 2- Hydrochloric acid (1 M): was prepared by taken 8.6 ml of concentrated HCl and diluted to 100 ml with distilled water. 4- Sodium hydroxide (6 M): was prepared by dissolving 24 g of NaOH in 100 ml of distilled water. Standard solution of CFX was prepared by dissolving accurately weighted amount of the pure drug equivalent to 10 mg in 1ml of concentrated HCl and further diluted to 100 ml with distilled water. The constructed calibration curve was used to compute the amount of CFX in the given samples

Results and discussion

Conclusions

Trihydrate and Cefuroxime Axetil in Bulk