Spectrophotometric determination of nevirapine using tetrathiocyanatocobalt(II) ion as a reagent

T V Sreevidya,B Narayana

doi:10.1590/s0100-46702010000300008

Abstract

A simple and rapid spectrophotometric method for the determination of nevirapine is described. The method is based on the reaction of nevirapine with tetrathiocyanatocobalt(II) ion in buffer of pH 4 to form the corresponding complex. Beer's law is obeyed in the range of 0.2 - 2.0 μg mL -1 for nevirapine. The optical parameters such as molar absorptivity, Sandell's sensitivity, detection limit and quantitation limit were found to be 1.16× 10 4 Lmol -1 cm -1 , 2.09 X 10 -3 μg cm -2 , 0.073 μg mL -1 and 0.222 μg mL -1 respectively. The optimum reaction conditions and other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision. The proposed method has been successfully applied for the determination of nevirapine in pharmaceutical formulations.

Highlights

Dipyrido[3,2-b:2′,3′-e][1,4]diazepin-6-one, is a non-nucleoside reverse transcriptase inhibitor (NNRTI) class of antiretrovirals used for the treatment of HIV-1 infections and AIDS [1,2,3]
Nevirapine in triple combination therapy has been shown to suppress viral load effectively when used as initial antiretroviral therapy [6,7]
The method involves the reaction of NVP with Tetrathiocyanatocobalt(II) ion (TTC) in pH 4 to form a complex [Scheme 1], which has an absorption maximum at 624.5 nm [Fig. 1]

Summary

Introduction

Nevirapine (NVP) chemically11-cyclopropyl-4-methyl-5,11-dihydro-6H-dipyrido[3,2-b:2′,3′-e][1,4]diazepin-6-one, is a non-nucleoside reverse transcriptase inhibitor (NNRTI) class of antiretrovirals used for the treatment of HIV-1 infections and AIDS [1,2,3]. Nevirapine is structurally a member of the dipyridodiazepinone chemical class of compounds. It is used in resource poor areas and it is available as a part in generic drug combinations. It is an inducer of cytochrome P450 isoenzymes CYP3A4 and CYP2B6 [4,5]. Nevirapine in triple combination therapy has been shown to suppress viral load effectively when used as initial antiretroviral therapy [6,7]. It is a potent and selective non-

Methods

Results

Conclusion