Abstract
A simple and sensitive spectrophotometric method was described for the determination of sulphacetamide sodium (SAS) in aqueous solution. The proposed method depends on diazotization of drug compound using sodium nitrite in acidic medium to produce the corresponding diazonium salt which coupled with 2,6-dihydroxytoluene reagent in presence of sodium carbonate to form a colored water soluble and stable azo-dye, which has a maximum absorption at 435 nm with a molar absorptivity and Sandalʼs sensitivity of 4.6×104 l.mol-1.cm-1 and 0.00543 µg.cm-2 respectively. Beer's law is valid over the concentration range 0.25-12.5 µg.ml-1. The relative error is in between -0.40 and +3.60 % and the relative standard deviation from ±0.13 to ±1.25 % depending on the concentration level. The proposed method was applied successfully to determine sulphacetamide sodium in its pharmaceutical formulation as ophthalmic drops.
Highlights
ABSTRACT A simple and sensitive spectrophotometric method was described for the determination of sulphacetamide sodium (SAS) in aqueous solution
The proposed method depends on diazotization of drug compound using sodium nitrite in acidic medium to produce the corresponding diazonium salt which coupled with 2,6-dihydroxytoluene reagent in presence of sodium carbonate to form a colored water soluble and stable azo-dye, which has a maximum absorption at 435 nm with a molar absorptivity and Sandal’s sensitivity of 4.6×104 l.mol-1.cm-1 and 0.00543 μg.cm-2 respectively
Beer's law is valid over the concentration range 0.25-12.5 μg.ml-1
Summary
ABSTRACT A simple and sensitive spectrophotometric method was described for the determination of sulphacetamide sodium (SAS) in aqueous solution. The proposed method depends on diazotization of drug compound using sodium nitrite in acidic medium to produce the corresponding diazonium salt which coupled with 2,6-dihydroxytoluene reagent in presence of sodium carbonate to form a colored water soluble and stable azo-dye, which has a maximum absorption at 435 nm with a molar absorptivity and Sandal’s sensitivity of 4.6×104 l.mol-1.cm-1 and 0.00543 μg.cm-2 respectively.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
