Abstract
A simple, rapid, accurate and precise spectrophotometric method is proposed for determining metoclopramide – hydrochloride (MCPH) in pure form and in pharmaceutical preparations .The method is based on oxidation - reduction reaction between metoclopramide - hydrochloride and cerium (IV) ion, then the subsequent reaction of cerium (III) and arsenazo III reagent in acidic medium to produce a blue-violet complex which is stable, water-soluble and has a maximum absorption at 654nm with a molar absorptivity of 9.36x104 l.mol-1.cm-1 and Sandell's sensitivity of 0.0038 µg.cm-2. Beer's law is obeyed in concentration range from 1-30 µg metoclopramide - hydrochloride in final volume of 25 ml, (0.04-1.2) ppm with a relative standard deviation (RSD) ( ±1.80% - ±3.98%) and the limit of detection (LOD) is 0.0095 µg/ml and the limit of quantitation (LOQ) is 0.0317 µg/ml . The method is suitable for the determination of metoclopramide - hydrochloride in the presence of other ingredients that are usually present in dosage forms and the recoveries are obtained in the range of 97.6-101.0 % . The proposed method has been applied successfully to the determination of the intended compound in its pharmaceutical preparations ( tablet and injection).
Highlights
A simple, rapid, accurate and precise spectrophotometric method is proposed for determining metoclopramide – hydrochloride (MCPH) in pure form and in pharmaceutical preparations .The method is based on oxidation - reduction reaction between metoclopramide - hydrochloride and cerium (IV) ion, the subsequent reaction of cerium (III) and arsenazo III reagent in acidic medium to produce a blue-violet complex which is stable, water-soluble and has a maximum absorption at 654nm with a molar absorptivity of 9.36x104 l.mol-1.cm-1 and Sandell's sensitivity of 0.0038 μg.cm-2
The drug was used for the control of sickness due to radiation therapy and chemotherapy, and for the prevention and treatment of postoperative nausea and vomiting (PONV)
The intensity of the formed complex has been found to be proportional to the amount of MCPH originally present in solution
Summary
A simple, rapid, accurate and precise spectrophotometric method is proposed for determining metoclopramide – hydrochloride (MCPH) in pure form and in pharmaceutical preparations .The method is based on oxidation - reduction reaction between metoclopramide - hydrochloride and cerium (IV) ion, the subsequent reaction of cerium (III) and arsenazo III reagent in acidic medium to produce a blue-violet complex which is stable, water-soluble and has a maximum absorption at 654nm with a molar absorptivity of 9.36x104 l.mol-1.cm-1 and Sandell's sensitivity of 0.0038 μg.cm-2. Metoclopramide was used as a treatment of nausea and vomiting in association with migraine and severe headache. In this setting, the combination of metoclopramide with an analgesic proved to be very efficacious with a short delay of action. The chromatographic methods are a lot cost, they are consuming of time and limiting of application and the flow-injection chemiluminescent are often typically less sensitive and have their own intrinsic disadvantages such as technical complexity or require an expensive instrumentation (Al-Arfaj, 2004)
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