Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

Khadiga M Kelani Abdallah A Shalaby

doi:10.4172/2153-2435.1000321

Abstract

In this study determination of Moexipril hydrochloride (MOX) and hydrochlorothiazide (HCT) were conducted by application of Spectrophotometric and Chemometric methods. Five different accurate, sensitive and reproducible methods were applied for the simultaneous determination of (MOX) and (HCT) in their bulk powder and pharmaceutical dosage form. The first method is the new absorbance subtraction (AS) method. The second method is the new amplitude modulation (AM) method. The third method is the new extended ratio subtraction (ERS) method coupled to ratio subtraction (RS) method. The fourth and fifth methods are multivariate calibration which includes Principal Component Regression (PCR) and Partial Least Squares (PLS). The suggested procedures were checked using laboratory prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparations. The validity of the proposed methods was further assessed by applying the standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with a reported method.

Highlights

Moexipril Hydrochloride (MOX) is chemically (3S)-2-[(2S)-2{[(2S)-1-ethoxy-1-oxo-4-phenylbutan-2- yl] amino} propanol]-6, 7-dimethoxy-1, 2, 3,4-tetrahydroisoquinoline-3-carboxylic acid, Figure (1)
By the analysis of the recorded absorbance at the isoabsorptive point, the absorbance corresponding to MOX or HCT, separately, at isoabsorptive point 210 nm can be calculated using absorbance factor [abs 210 / abs 310] which is the average of the absorbance of different concentrations of pure HCT using isoabsorptive point at 210 nm to that at 310 nm which shows no contribution of MOX and the absorbance of MOX can be obtained after subtraction
This study introduces five different methods for determination of MOX and HCT in their binary mixtures

Summary

Introduction

Moexipril Hydrochloride (MOX) is chemically (3S)-2-[(2S)-2{[(2S)-1-ethoxy-1-oxo-4-phenylbutan-2- yl] amino} propanol]-6, 7-dimethoxy-1, 2, 3,4-tetrahydroisoquinoline-3-carboxylic acid, Figure (1). HCT is a first-line diuretic drug of the thiazide class used as antihypertensive drug that acts by inhibits active chloride reabsorption at the early distal tubule via the Na-cl co transporter, resulting in an increase in the excretion of sodium, chloride, and water. This reduces the volume of the blood, decreasing blood return to the heart and cardiac output and, by other mechanisms, is believed to lower peripheral vascular resistance. HCT is official in The British Pharmacopeia [5], The European Pharmacopeia [6] and the United States Pharmacopoeia [7]

Methods

Results

Conclusion