Abstract
The content of fatty acid (FA) triglycerides in food and biological media is predicting traditionally using gas and liquid chromatographic methods. Named techniques aren’t available for clinical labs due to their complexity. So, our objective was to develop the method and apparatus for rapid assay of a few clinically important FA as the saturated palmitic, mono unsaturated oleic and others in serum using near infrared spectrometer. As a result, the applicability of the FT spectrometer in the wavelength range of 0.9 -1.8 μ to analyze these FA in serum without sample preparation was confirmed. Besides, measurement specifications were determined and a correlations of the absorption spectra and contents of total triglycerides and cholesterol, palmitic, oleic, linoleic and arachidonic FA in serum were established
Highlights
It is well known [1,2] that cardiovascular diseases and cancer - the main causes of human death, are statistically associated with an increased content of saturated fatty acid triglycerides (FA) compared to unsaturated FA in the blood
For the present study three sets of 27, 30 and 42 serum samples taken in different periods were selected from different patients of the clinical department of Russian Cardiology Research Complex
The individual FA triglycerides content were quantifocated with extraction, separation and methylation of FA with the use of HPLC DIONEX Ultimate 3000 using
Summary
It is well known [1,2] that cardiovascular diseases and cancer - the main causes of human death, are statistically associated with an increased content of saturated fatty acid triglycerides (FA) compared to unsaturated FA in the blood. The usage of near infrared spectroscopy, combined with the method of multi-component calibration - regression on projections to latent structures (PLS), seems promising for mass express-control of the above-mentioned FA in serum of patients [3].
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