Abstract
Abstract. This study examined the absorption and fluorescence spectra of chlorophylls and other pigments extracted in ethanol solutions from common edible plant species. Chlorophyll-a was the dominant fluorescent chromophore in all plant varieties. The fluorescence curves for all extracted solutions exhibit after optimizing by concentration dilution an increase in the fluorescence with a typical prominent leading Gaussian peak at a wavelength of 674 nm. This peak is followed by a decreasing shoulder extending into the near-infrared range beyond 724 nm. The dilution of the solutions with ethanol reduces the optical absorption from the other pigments and shows simultaneously an improved resolution of the chlorophyll-a spectral absorption peaks. This investigation provides new insights into the optical absorbance and other photo-excited processes that impact the fluorescence of chlorophylls, such as agglomeration, photobleaching and photo-aggregation. The findings may be used for biodiagnostics, fluorescent quantum dots and bio-sensors.
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