Abstract
Nitrocide spin labels containing 15N have hyperfine lines at magnetic field positions different from those for 14N spin labels. This allows the use of an 14N and an 15N spin label for specific experiments where two events can be monitored at the same time. Before this technique can be used to full advantage, the spectral characteristics of 15N spin labels under various conditions must be known. This report describes, in a comparative way, the effects of restricted rotational motion and nitroxide—nitroxide interactions on 15N-Temponë and the corresponding 14N spin label. An interesting feature is that 15N extends the useful range of motion of spin labels to slower tumbling rates than those conveniently handled with 14N. A general description of line shape changes due to spin label concentration effects is made without resorting to formalism.
Published Version
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