Abstract
Interactions between asialofetuin (AF)-labeled liposomes (AF-liposomes) and hepatocytes were investigated in vitro. The hepatocytes were isolated from rat liver by the collagenase perfusion method. Labeling the surface of liposomes with AF enhanced the hepatocyte uptake of liposomes consisting of phosphatidylcholine, phosphatidic acid and cholesterol. The uptake of AF-liposomes (cholesterol-labeled) by hepatocytes decreased on adding AF, and virtual total inhibition was attained with the addition of unlabeled AF-liposomes. The enhancing effect of AF on liposome uptake increased with liposomal cholesterol content. The membrane fluidity of AF-liposomes decreased with increasing cholesterol. From these results, there appears to be the possibility that AF-liposomes are specifically incorporated into hepatocytes through the action of galactose-binding protein.
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