Specific TCD8+IL-10+ and TCD8+TGF-beta+ cells in active tuberculosis patients

Abstract

Event Abstract Back to Event Specific TCD8+IL-10+ and TCD8+TGF-beta+ cells in active tuberculosis patients Bruna Daniella S. Silva1*, Monalisa M. Trentini1, Adeliane C. Costa1, Abadio O. Costa-Júnior1, André Kipnis1 and Ana Paula Junqueira-Kipnis1 1 Universidade Federal de Goiás, Departamento de Microbiologia, Imunologia, Patologia e Parasitologia, Brazil Tuberculosis (TB) is responsible for causing over 1.5 million deaths each year. TCD4+ together with TCD8+ cells and their cytokines are important for protective immune response to TB. Although TCD8+ cells has been studied in TB, several aspects is not yet clarified. In the absence of TCD4+ cells, TCD8+ cells increase their cytotoxic function and production of different cytokines in order to respond to Mtb infection. To understand the role of TCD8+ cells as regulatory cytokine producers in TB, peripheral blood TCD8+ cells were characterized and compared their profile with healthy controls (TST-). PBMC from 15 patients with active pulmonary TB (mean age = 40.5) and 15 healthy controls TST-, matched by sex and age to TB patients, were cultivated for 96 hours with Mtb recombinant antigen (rMPT-51). TCD8+ cell subsets were analyzed by flow cytometry. TB patients presented higher levels of TCD8+IL-10+ (medium=1.2±0.9; PHA=12.8±1.5; rMPT-51=5.0±1.4) and TCD8+TGF-beta+ (medium=1.6±1.3; PHA=14.1±1.7; rMPT-51=8.0±1.1) in response to MPT-51 than TST- individuals (TCD8+IL-10, medium=1.3±1.1; PHA=11.9±1.7; rMPT-51=3.0±1.5); (TCD8+TGF-beta+, medium=2.4±0.8; PHA=13.6±2.3; rMPT-51=2.8±1.2). The cytotoxic markers of TCD8+ cell such as Perforin and Granzime-B are independent of the MPT-51 stimulus, and TB patients had lower percentage of triple positive cells than the healthy controls (TST-=21.7±2.8; TB=9.1±1.3). Conclusions: Patients with TB presented Mtb specific TCD8+ positive for IL-10 and TGF-beta, whereas presented lower cytotoxic markers. These results suggest that TCD8+ cells can contribute to the regulatory immune response in active tuberculosis. Acknowledgements Financial Support: CNPq; CAPES. Keywords: Tuberculosis, TCD8+ cells, rMPT-51, cytotoxic activity, regulatory cells Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Silva BS, Trentini MM, Costa AC, Costa-Júnior AO, Kipnis A and Junqueira-Kipnis A (2013). Specific TCD8+IL-10+ and TCD8+TGF-beta+ cells in active tuberculosis patients. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00094 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Bruna Daniella S Silva, Universidade Federal de Goiás, Departamento de Microbiologia, Imunologia, Patologia e Parasitologia, Goiânia, Goiás, 74605050, Brazil, bruna.daniella@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Bruna Daniella S Silva Monalisa M Trentini Adeliane C Costa Abadio O Costa-Júnior André Kipnis Ana Paula Junqueira-Kipnis Google Bruna Daniella S Silva Monalisa M Trentini Adeliane C Costa Abadio O Costa-Júnior André Kipnis Ana Paula Junqueira-Kipnis Google Scholar Bruna Daniella S Silva Monalisa M Trentini Adeliane C Costa Abadio O Costa-Júnior André Kipnis Ana Paula Junqueira-Kipnis PubMed Bruna Daniella S Silva Monalisa M Trentini Adeliane C Costa Abadio O Costa-Júnior André Kipnis Ana Paula Junqueira-Kipnis Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.