Abstract
In modern botanical studies, various molecular genetic methods such as genome sequencing, PCR, AFLP-analysis, etc. are often involved. These methods require the use of high-quality (i.e. well purified and non-degraded) genomic DNA. However, extraction of such DNA from plants is complicated by a wide spectrum of organic compounds that contaminate DNA and drastically reduce its quality. As a result, the protocols for DNA extraction from plants are usually labor-intensive, time-consuming and require expensive reagents, most of which are imported from abroad. In the case of high-throughput DNA extraction from plant material, these disadvantages are of a great importance, especially in view of the current import problems. Moreover, there is no universal protocol suitable for all plant species and all variants of plant material used: different protocols are effective in different cases and additional modifications are often required. Promising ways to overcome these problems include the search for simplified methods of plant DNA extraction, as well as the use of specially prepared initial material.
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