Specific phase expression of Pax3 in excessive retinoic acid teratogenicity and taurine intervention on the neural tube defects

Abstract

Objective To investigate the excess retinoic acid (RA) teratogenesis and taurine intervention in the embryonic neural tube defects(NTDs),and analyze the Pax3 specific phase expression in NTDs.Methods Feed excess retinoic acid and taurine to pregnancy mice and set up the models of the fetal neural tube defects and intervention mice.Use the methods of immunohistochemistry and reverse transcription-polymerase chain reactor to analyze the Pax3 specific phase expression in each group of E9.5,E10.5,E11.5,E12.5 fetal rats during neural tube development.Results The malformation rate in teratogenic group is 71.6％,while in intervention group it drops to 55.3％ (P ＜ 0.05).The rates of the stillbirth and absorption of the two groups were 11.4％ and 9.8％ (P ＞0.05) ;the Pax3 protein positive immunostaining mainly located on the nucleus; the expression of Pax3 protein in the neural crest is stronger than those in the mesenchyme.Pax3 protein expression in the control group is stronger than those in the two experimental groups (P ＜ 0.05),and the peak expression appeared at E10.5.Among the two experimental groups,Pax3 expression showed increasing trend,and its expression in the intervention group is stronger than the teratogenic group (P ＜ 0.05).The Pax.3 gene expression is inhibited in the two experimental groups,is significantly decreased comparing with the control group (P ＜ 0.05),and the peak expression of Pax3 gene appeared at E10.5 in the control group; Among the two experimental groups,Pax3 expression showed increasing trend with the embryos development,and in the intervention group,it is stronger than the teratogenic group (P ＜ 0.05).Conclution The role of retinoic acid teratogenic and taurine intervention on the NTDs development was associated with the Pax3 gene specific phase expression. Key words: Neural tube defects; Retinoic acid; Taurine; Pax3; Kunming mouse