Abstract
in the airways of sLTR and to compare this to BOS and non-transplant control airways. Methods: BAL and endobronchial biopsy (EBB) were obtained from clinically sLTR (n 10), LTR with BOS (n 10) and normal controls (n 10). BAL cell counts, differential cell counts and T-lymphocyte subsets were analysed by flow cytometry. IL-11 & 17 were detected in EBB tissue by use of immunohistochemistry & in situ hybridisation, and quantified as positive cells/mm2 of lamina propria with computer image analysis. Immuno-stains for neutrophils and lymphocytes were also performed on the EBB sections. Results: Data are shown in the table below. IL-11 expression was increased in the airways of sLTR in comparison to those with BOS (p 0.001) and controls (p 0.05), whereas overexpression of IL-17 was present in BOS airways (p 0.001). No significant correlation was found between IL-11 or IL-17 with airway or BAL inflammatory cell counts. Conclusion: These results revealed a significant differential up-regulation of IL-11 &17 between sLTR and LTR with BOS. A longitudinal study of IL-11 & 17 expression in lung allograft airways may offer unique insights into BOS pathogenesis post lung transplantation.
Published Version
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