Abstract
8-iso-Prostaglandin F 2α (8-iso-PGF 2α) is currently discussed as a potential index parameter of oxidative stress in vivo. We describe in this article a fully validated gas chromatographic–tandem mass spectrometric method for the quantitative determination of 8-iso-PGF 2α in human urine. The method is highly specific and requires a single thin-layer chromatographic step for sample purification. Inter- and intraday imprecision were below 8%. Mean inaccuracy was 5.3% for added levels of 8-iso-PGF 2α up to 2000 pg/ml of urine. We measured highly elevated excretion of 8-iso-PGF 2α in the urine of children with peroxisomal β-oxidation deficiency, i.e. Zellweger syndrome, (63.3±16.6 ng/mg creatinine) compared to that of healthy children (0.51±0.16 ng/mg creatinine) (mean±S.D., both n=5). The method could be useful for diagnosing Zellweger syndrome and for investigating the utility of 8-iso-PGF 2α as a novel marker for oxidative stress in vivo in man.
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