Specific and Non-Specific Parameters Standardization of Ethanolic 96% Extract of Kersen Leaves (Muntingia calabura L.)

Abstract

(Muntingia calabura L., commonly known as “Kersen” in Indonesia, which is a plant that grow in anywhere. Kersen leaves (Muntingia calabura L.) has been used empirically as a medicinal plant because it contains many potential compounds. This study aims is to standardize and Phytochemical screening the ethanolic 96% extract of kersen leaves (Muntingia calabura L.). Standardization of the ethanolic 96% extract of kersen leaves (Muntingia calabura L.) consist of two parameters that is specific and non specific. The specific parameters include organoleptic test, water and ethanol extractable material, identification of compound content. Whereas non specific parameter include water content, density extract, acid insoluble ash content, mold & yeast contamination, total ash content, microbial contamination, and metal contamination such as Pb and Cd. The result showed that the organoleptic properties of ethanolic 96% extract of kersen leaves (Muntingia calabura L.) are dark green color, distinctive smell and has a slightly bitter taste. Content of water solvent and ethanol solvent of the ethanolic 96% extract of kersen leaves is 60.67% and 12.1%. Phtyochemical screening results of the ethanolic 96% extract of kersen leaves contains saponins, fenol, flavonoid, steroid. The non specific parameters of the ethanolic 96% extract of kersen leaves are water content of 8.88%; density extract of 0.815 gr/mL, total ash content of 2.27% ± 0.15; acid insoluble ash content 0.05% ± 0,04; mold and yeast contamination of <1.0 x 100 CFU/gr; microbial contamination of <1.0 x 100 CFU/gr; Pb level of 0.07 ppm ± 0.03 and Cd level of <0.001 ppm. Based on these result that the ethanolic 96% extract of kersen leaves (Muntingia calabura L.) has met the predetermined requirements.