SPECIFIC ACTIVITY OF BIOLOGICALLY ACTIVE COMPLEX IN LIQUID HERBAL DRUG EXTRACTS, STUDIED UNDER DIFFERENT DRYING CONDITIONS

Abstract

The aim of this study was to evaluate the influence of different methods of extraction solvents removal from liquid herbal drugs extracts on their pharmacological activity (immunomodulating, adaptogene, antioxidant and antibacterial). The authors used specific enzyme biotest systems in vitro based on key enzymes of antioxidant protection system - glutathione reductase and catalase, as well as on limiting enzyme of phagocytosis terminal phase – NADPH-oxidase, that allow the researchers to identify adaptogene, antioxidant, antibacterial and immunomodulating BAS activity and to estimate possible alterations in bioactivity of the studied objects. The obtained results showed that the drying temperature of liquid combined herbal drugs extracts does not influence significantly on its BAS complex bioactivity. Extract samples, dried at different temperatures, have immunomodulating and antibacterial activity. Best results were shown by the two samples: 1) dried at t=60 ± 1°С without vacuum application, and 2) preliminary adsorbed on the mix of powder like excipients isomalt GalenIQ® and Aeroperl® 300 Pharma, and then dried at room temperature. The materials of this article can be useful for specialists in pharmaceutical technology, biochemistry, and pharmacology. The obtained results contribute to the development of new advanced drug formulations based on BAS of herbal drugs extracts.