Abstract
Confocal microscopy, one of the modern day fluorescence laser-scanning microscopic techniques, is suffered from having sufficient out-of-focus signal. On the other hand, in multiphoton microscopy ultrafast laser pulses are commonly used to circumvent low multiphoton absorption cross-sections of common fluorophores; due to broad overlapping two-photon absorption (TPA) spectra of fluorophores and large spectral bandwidth of a short pulse, simultaneous excitation of many fluorophores is common demanding selective excitation of individual fluorophores if required.
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