Abstract
Current advances in fluorescence-based superresolution imaging have illustrated great details of cellular structures that are invisible to conventional fluorescence light microscopy. The significantly improved spatial resolution is especially advantageous for bacterial cells because of their small sizes. In the past few years the spatial organization and dynamics of a variety of bacterial cellular structures and protein macromachineries have been revealed with unprecedented details. As the field matures, it is now time to focus on the functional aspect of the observed spatial organizations and dynamics. Are they essential in carrying out a specific cellular function? Do they play a regulatory role in controlling the on and off of a certain cellular process? In this work I will present a few examples from our laboratory that examine the spatial and functional organization of macromolecules involved in bacterial cell division and transcription. We combine superresolution imaging with biochemical activity detection to illustrate the spatial organization-function relationship for E. coli RNA polymerase and the cytokinesis FtsZ-ring.
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