Soybean Isoflavones Activating Autophagy and Improving the Chemosensitivity of Carboplatin to Ovarian Cancer Cells

Abstract

Objection: To discuss soybean isoflavones activating autophagy and improving the chemosensitivity of carboplatin to ovarian cancer cells. Materials and Methods: Using SKOV3 and A2780 cell lines as research object, dividing into Normal, Carb (treated with 50 μmol/L carboplatin) and Carb+Soy (treated with 50 μmol/L carboplatin and 80 μmol/L soybean isoflavone). Evaluating cell proliferation by EdU and MTT assay; measuring cell apoptosis rate by flow cytometry; observation cells’ autophagy by transmission electron microscope (TEM); LC 3B protein expression were evaluated by cellular immunofluorescence and using WB assay to evaluate autophagy relative proteins expression. Results: Compared with Normal group, the cell proliferation were significantly depressed with cell apoptosis rates significantly increasing (P < 0.01, respectively); and autophagy enhancing; with LC 3B, LC 3II/LC 3I ratio and Beclin 1 significantly up-regulation and P62 protein significantly down-regulation (P < 0.01, respectively) in Carb and Carb+Soy groups. And there were significantly differences between Carb and Carb+Soy groups in EdU cell number, cell proliferation, apoptosis rate, autophagy, LC 3B, LC 3II/LC 3I ratio, Beclin 1 and P62 protein expression. Conclusion: Soy activating autophagy and improving the chemosensitivity of carboplatin to ovarian cancer cells.