Soyabean Oil Supplementation Effects on Perivascular Inflammation in Lungs Induced by Bisphenol A: A Histological Study.

Abstract

To determine the effect of soyabean oil supplementation on perivascular inflammation in lungs of adult mice induced by Bisphenol A(BPA). An experimental study. Department of Anatomy, Army Medical College, Rawalpindi, in collaboration with the Animal House, National Institute of Health, Islamabad, from June to November 2016. Thirty male and female BALB/c mice were divided into three groups, of 10 animals each. Group Aanimals served as control. Group B animals were given BPAat a dose of 50 mg/Kg body weight/day. Group C animals were given BPAand soyabean oil at doses of 50 mg/Kg body weight/day and 500 mg/day, respectively. All treatments were given once daily for a period of eight weeks. Animals were dissected 24 hours after receiving the last dose. Lung tissue specimen processing and H&E staining was carried out for routine histological study. Perivascular inflammation was morphometrically graded and statistically analysed using Chi-square test with p<0.05. Grade 2 inflammation was recorded in two (20%) animals and grade 3 perivascular inflammation in 80% specimens in Group B; whereas 20% specimens of Group C had grade 2 inflammation and eight (80%) showed grade 1 inflammation. None of the control animals showed any inflammation. All groups were significantly different at p<0.001. BPAproduced perivascular inflammation and con-commitant administration of soyabean oil diet protected against it in rodent.