Source of the increased serum alkaline phosphatase activity in paget's disease

Abstract

The serum of five patients with Paget's disease was tested with an antiserum which would react with alkaline phosphatase isolated from human liver and granulocytes, but not with alkaline phosphatase from bone. The results of the study indicated that 90% of the total serum alkaline phosphatase activity of these patients was removed by this antiserum. Since this antiserum did not react with a bone alkaline phosphatase preparation, these observations suggest that in this disease the rise in serum enzyme activity is not from an increase in the concentration of the bone isoenzyme as has been postulated, but is probably from the liver isoenzyme.