Abstract
SHORTLY after the development of dextran gels as packing material for size separation of solutes by gel chromatography (gel filtration)1, the sorption properties for low molecular weight aromatic substances were observed2. In spite of numerous investigations, some of which compared molecular structure with degree of retardation, and some of which exploited the side effects of gel chromatography for actual separation problems3, no interpretation was given for these rather strong interactions. Aromatic and heterocyclic substances are sometimes retarded during liquid chromatography on various sorbents such as charcoal, cellulose, silica, alumina oxide and polyacrylamide3, but the effects are much less pronounced than in the case of ‘Sephadex’ dextran gels. The sorption properties of ‘Sephadex’ gels should be divided into two classes: (a) the “mass action” and (b) special “binding” forces between solutes with π-electrons and the ‘Sephadex’ matrix. It is the nature of this specific binding that we wish to describe.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
