Source Mechanisms for Unit Activity in Isolated Crayfish Central Nervous System

Abstract

As a tool for the identification of source mechanisms underlying the activity of single interneurons in the isolated crayfish abdominal cord, pulse trains from a sample of such neurons are statistically described (shape, mean, standard deviation, and serial correlation coefficient of interval distributions). These statistics were measured under four independent means of obtaining different average frequencies: (a) naturally occurring frequencies under standard conditions, (b) temperature control, (c) dc polarization, and (d) electrical stimulation of presynaptic fibers. 40 of 44 units studied had unimodal histograms, symmetrical when the mean interval was small and positively skewed with large means. Under standard conditions these units had SD = k (mean)(n), with n approximately 2. Fifteen single units were caused to fire at varied frequencies by cooling or dc stimulation. The resulting SD-vs.-mean plots for single units showed: (a)all points for a given unit fell approximately on a single line, regardless of whether temperature, dc, or neither was used to vary frequency, and (b) the average value of n (slope of log SD vs. log mean) for the fifteen units was 1.9 +/- 0.2. A paradox arises from interpretation of these data via gamma distributions. It is concluded that most of the spontaneous activity in the isolated abdominal cord may result from pacemaker activity within each cell and does not require a network of active units. Finally, the fact that the SD-mean relation was found not to depend measurably on temperature is interpreted as a useful restriction on models for neuronal noise processes.