Abstract
The proportion of the noninfectious nucleoprotein with a normal amount of RNA (B 2 fraction) found in turnip yellow mosaic virus (TYMV) preparations following fractionation in CsCl density gradients, increased from about 15% to 50% as the pH of the CsCl solution was raised from 7.0 to 8.5. The other minor nucleoprotein fractions were not increased in this way. When the pH of the CsCl was controlled in the range 5.0–7.0, and the SW 40 rotor was used, double bands of each of the previously described nucleoprotein components were revealed. Thus ten nucleoprotein bands could be observed, two each for B 000, B 00, B 0, B 1, and B 2. The less dense band ( a band) of each pair was present in greater amounts. Both the bands found in the component previously shown to contain infectivity (B 1) were infectious. Mixing these isolated bands (B 1a and B 1b) did not enhance infectivity. Double bands were formed by some preparations of empty protein shells when these were subjected to centrifugation in CsCl of D = 1.27 g/cm 3. No consistent differences were found in the behaviour of the polypeptide from a and b bands on polyacrylamide gel electrophoresis.
Published Version
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