Abstract
1. 1. A soluble Mg-dependent ATPase, similar to the mitochondrial ATPase from beef heart, has been isolated from heart mitochondria of salmon ( Salmo salar). The salmon heart ATPase has 5 subunits with molecular weights similar to the beef heart enzyme, but the Stoke's radius of the intact salmon enzyme is larger. 2. 2. The salmon heart ATPase is less temperature labile than the beef heart enzyme. 3. 3. The salmon heart ATPase is strongly inhibited by ADP, and the inhibition is highly temperature dependent. The ITPase activity is also inhibited by IDP ( K i = 180 μm). 4. 4. 2,4-Dinitrophenol in small concentrations stimulates the ITPase activity as well as the ATPase activity of the “washed” salmon heart enzyme. However, in an enzyme preparation which had been freed of most of the bound nucleotides by dialysis in the presence of glycerol (Roveri et al., 1980) the ITPase activity is not stimulated by 2,4-dinitrophenol.
Published Version
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