Abstract
Summary In vitro morphogenesis of Arabidopsis callus cultures is shown to be under the control of defined hormonal ratios and concentrations. Low kinetin concentrations (10 -7 M) are desirable during callus induction to obtain subsequent organ differentiation. Leaf formation was optimal with a ratio 10 -6 M kinetin/10 -7 M IAA. Root formation from the callus occured on a wide range of cytokinin/auxin ratios. The importance of a defined sequence of culture media to establish an optimal hormonal balance for leaf formation is discussed. Ageing of the callus in culture is accompanied by a progressive decline of the morphogenetic response and by an increase of the degree of ploidy.
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