Some Enzymatic Changes in Ischemic and Hydronephrotic Rat Kidneys

Abstract

1) All the four enzymes stained and fat staining showed marked differences between 1 1/2 and 2 1/2hrs. of occlusion.2) Both ischemic and hydronephrotic kidneys revealed dilated tubules. Some of them showed low emzymatic activity, while the others remained to possess normal enzymatic activity. The latters are considered to be hypertrophied to compensate necrotic or low functioning tubules.3) The significance of increased I.R. and S.D. activities in the ischemic kidneys is not known.4) Marked differences were observed in the enzymatic activities between hydronephrses produced by ureteral ligation of less than 10 days and longer than 20 days. This fact may contribute to know whether or not renal tissue is viable.5) The fact that S.D. and TPN activities increased in the medulla and decreased in the cortex is interesting, when the experiment with Na24 showing higher concentration in the medulla is referred.6) No changes of enzymatic activities were seen in the contralateral kidneys in hydronephrotic and ischemic cases. This means that the healthy kidneys contain a certain amount of enzymes enough to compensate the decreased function of the treated kidneys. Renal functional reserve is accompanied by renal enzymatic reserve.7) Fatty degeneration was observed in slightly dilated tubules hydronephrotic and ischemic kidneys. It suggests that fatty changes occurred at the beginning of cellular degenration.