Abstract
Anomalous water samples were prepared by condensing water vapor into glass and quartz capillaries. The transfer of microliter quantities of anomalous water/water mixtures into larger capillaries suitable for further studies was accomplished by centrifugation. Some of the droplets showed optical anisotropy when viewed under a polarizing microscope. A density gradient method was developed to measure the specific gravity of anomalous water droplets. In anomalous water the rate of trypsincatalyzed hydrolysis of the chromogenic substrate N α -benzoyl-DL-arginine p-nitroanilide was less than 10% of the rate of hydrolysis observed in normal water. As reported earlier, the state of water associated with the enzyme carbonate hydro-lyase (EC 4.2.1.1) was studied by means of γ-γ directional correlation. With the use of the same technique and 133Ba tracer, a method was developed to measure and compare relaxation times and field symmetries in normal water, protein-bound water, and anomalous water condensed in capillaries.
Published Version
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