SOME BIOCHEMICAL AND HAEMATOLOGICAL STUDIES IN BRUCELLOSIS INFECTED BUFFALOES, COWS AND SHEEP IN EL-SHARKIA GOVERNORATE

Abstract

Blood samples from 351 buffaloes, 149 cows and 551 sheep were takenfrom a farm at New Salhia, (El-Sharkia Governorate) and examinedfor the incidence of brucellosis by the following serological tests,qualitative buffered acidified plate antigen (BAPAT), Rose BengalPlate test (RBPT), the standard quantitative tube agglutination test(SAT) and the confirmatory quantitative complement fixation test(CFT). Biochemical and haematological studies were done on 60seropositive (20 from each species) as well as 60 seronegtaiveanimals (20 from each species) as control onesThe bioechmiacl analysis revealed a significant increased enzymaticactivities for both, asparatate aminotransferase (AST) and alanineaminotransferase (ALT), and a significant increase in urea level ofinfected animals as compared with the control ones. On the otherhand, a significant decrease was recorded in total serum protein andglucose levels in all infected species. Serum iron level showed asignificant decrease in infected cows only. Whereas, no changes weredetected for both serum calcium and inorganic phosphorus levels ininfected animals comparing with control ones.Concerning haematological profile, we recorded a significant decreasein Hb and PCV% in all infected species accompanied with a generaldecrease in RBCs count, only of significant value in infected cows. TheWBCs count showed insignificant alterations in all infected animalscompared with control ones.