Abstract
The article presents studies of organogenesis peculiarities of varieties belonging to various species of Amelanchier Medik. genus in in vitro culture at the stages of micropropagation, rooting and depositing. Mineral composition and growth regulator for active adventive microshoots formation were determined. The optimal auxin source for rhizogenesis of various Amelanchier alnifolia varieties has been established. Conditions for explants' preservation in aseptic conditions for 12 months have been revealed. Different effects of cytokinin type substances on shadberry regeneration potential under aseptic conditions were noted. Analysis of regenerants' morphometric indicators showed the effectiveness of using 6-benzylaminopurin (BAP) in the concentration of 1.0 mg/L for shadberry varieties' clonal micropropagation and indolebutyric acid (IBA) at 1.0 mg/L concentration for microshoots' rooting.
Highlights
Every year, the need for high-yield fruit and berry plantations resistant to various environmental factors is constantly increasing
Quorin and Lepoivre (QL) (1977), Woody Plant Medium (WP) (1980) and MurashigeSkoog (MS) (1962) were tested to determine the optimal mineral composition of the nutrient medium, the latter was used as control
Explants' regeneration features in varieties belonging to various species of Amelanchier genus have been identified
Summary
The need for high-yield fruit and berry plantations resistant to various environmental factors is constantly increasing. Root offspring and bush division allow to get no more than 6 daughter plants [12] This makes these methods ineffective for breeding shadberry varieties. Micropropagation of four varieties of A. alnifolia species revealed the application effectiveness of the nutrient medium Murashige-Skoog (MS) in comparison with Gamborg (1968), Nitsch (1968) and advantage of BAP over other sources of cytokinin (thidiazuron, zeatin and kinetin) [13, 14]. Such physiological disturbance as vitrification was studied on the example of A. arborea. The purpose of the scientific work was to identify morphogenesis features and compare the regeneration potential of Amelanchier genus representatives at the stages of micropropagation and rooting depending on used mineral compositions of nutrient media and growth regulators
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