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Abstract
Retention behavior and column efficiency in capillary electrochromatography (CEC) were compared with those in micro-high-performance liquid chromatography (μ-HPLC). Using a unified microcolumn separation apparatus, capacity factors of 27 neutral solutes in pressurized electrochromatography (PEC), CEC and μ-HPLC were investigated and no significant differences were found among these three modes. By linear solvation energy relationship analysis, the same linear equations were obtained in CEC, PEC and μ-HPLC. Systematic investigation of the retention behaviors under mobile phases with four different kinds of organic solvents showed that some of the retention rules in HPLC can be applied in CEC for neutral solutes. Gradient elution of ketones and aldehydes is discussed.
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