Abstract

Summary Somatic embryogenesis was induced in several cultivars of tomato (Lycopersicon esculentum Mill.) using a simple, one-step method. Seeds were germinated in the presence of 50 or 80 μmol · L-1 N6-benzylaminopurine, and the resultant seedlings were maintained for six weeks on the same medium. Somatic embryogenesis occurred directly without an intervening callus proliferation phase from the meristematic centres formed in the subepidermal region of the nodular tissues developed at the basal ends of induced seedlings. Regenerated somatic embryos were successfully grown into whole plants.

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