Abstract
A novel system for plant regeneration from hypocotyl protoplasts of Brassica juncea cv Leaf Heading and India Mustard was developed. Protoplasts divided rapidly and gave rise to pro-embryos at frequencies of 3–6% for Leaf Heading and 15–20% for India Mustard after 17 days on Murashige & Skoog's medium supplemented with 6% glucose and benzyladenine (BA) in combination with 2,4-dichlorophenoxyacetic acid each at 1 or 2.5 μM. Abundant plantlets were regenerated from embryogenic cultures of India Mustard grown on hormone-free medium, whereas regeneration of Leaf Heading was low. The presence of AgNO 3 at 30 μM in conjuction with 10 μM BA and 2.7 μM naphthaleneacetic acid considerably enhanced plant regeneration of Leaf Heading. After acclimatization all protoplast-derived plants of India Mustard and 91% of Leaf Heading were phenotypically normal.
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