Abstract
Background and Aim:Mastitis is a serious disease of dairy animals causing great economic losses due to a reduction in milk yield as well as lowering its nutritive value. The application of somatic cell count (SCC) and alkaline phosphatase activity in the milk for diagnosis of mastitis in buffalo is not well documented. Therefore, the present study was conducted to observe the SCC and alkaline phosphatase activity for evaluation of mastitis in buffalo.Materials and Methods:Milk samples of forty apparently healthy lactating buffaloes were selected and categorized into five different groups viz. normal buffaloes, buffaloes with subclinical mastitis with CMT positive milk samples (+1 Grade), (+2 Grade), (+3 Grade), and buffaloes with clinical mastitis with 8 animals in each group. The milk samples were analyzed for SCC and alkaline phosphatase activity.Results:The levels of SCC (×105 cells/ml) and alkaline phosphatase (U/L) in different groups were viz. normal (3.21±0.179, 16.48±1.432), subclinical mastitis with CMT positive milk samples with +1 Grade (4.21±0.138, 28.11±1.013), with +2 Grade (6.34±0.183, 34.50±1.034), with +3 Grade (7.96±0.213, 37.73±0.737) and buffaloes with clinical mastitis (10.21±0.220, 42.37±0.907) respectively, indicating an increasing trend in the values and the difference observed among various group was statistically significant.Conclusion:In conclusion, the results of the present study indicate that the concentration of milk SCC and alkaline phosphatase activity was higher in the milk of buffaloes with mastitis than in the milk of normal buffaloes.
Highlights
Mastitis is a serious disease of dairy animals causing great economic losses due to a reduction in milk yield as well as lowering its nutritive value
In conclusion, the results of the present study indicate that the concentration of milk somatic cell count (SCC) and alkaline phosphatase activity was higher in the milk of buffaloes with mastitis than in the milk of normal buffaloes
SCC (×105 cells/ml) and alkaline phosphatase activity (U/L) of milk from normal buffaloes and the buffaloes affected with subclinical and clinical mastitis are presented in Table-1, the same is depicted graphically in Figure-1 (SCC) and Figure-2
Summary
Mastitis is a serious disease of dairy animals causing great economic losses due to a reduction in milk yield as well as lowering its nutritive value. Somatic cells are mainly milk-secreting epithelial cells that have been shed from the lining of the gland and white blood cells (leukocytes) that have entered the mammary gland in response to injury or infection [3]. Alkaline phosphatase is an enzyme that is naturally found in milk The presence of this enzyme in the milk at levels elevated above normal suggests an increase rate of tissue destruction. The present study was taken up because the work on somatic cell count (SCC) and alkaline phosphatase activity in milk of normal and mastitic buffalo is not well documented. The application of somatic cell count (SCC) and alkaline phosphatase activity in the milk for diagnosis of mastitis in buffalo is not well documented. The present study was conducted to observe the SCC and alkaline phosphatase activity for evaluation of mastitis in buffalo
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