Abstract
The most common baseline anomalies observed in gradient elution high-performance liquid chromatography can be explained by the non-ideal behaviour of the solvents used. The bulk and molecular properties of mixed solvents, as they affect the perfomance of the liquid chromatograph, are explored. The concept of mole flow-rate, useful in heat balance and partition mechanism studies, is introduced and put to use in a preliminary heat balance study which could be sued to size heat exchange equipment useful in thermostatting a liquid chromatograph. The inter-dependecne of these properties is shown to produce cumulative effects in the pumping system, the column and the detector of the liquid chromatograph. The properrties of water-acetonitrile mixtures are used for illustration.
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