Abstract
The use of gradient elution high-performance liquid chromatography (HPLC) has imposed more stringent requirements for purity upon the solvents which will be used in these experiments. It has been made clear that acetonitrile has a greater native absorbance than water in the short-wavelength UV region (190–260 nm), but this alone cannot account for the baseline disturbances which many chromatographers observe when using this solvent. Furthermore, this phenomenon could not account for the baseline disturbances encountered when acetonitrile is used with fluorescence detection or with some electrometric detectors. We investigated the behaviour of several lots of acetonitrile by UV—HPLC UV absorbance spectroscopy and pH, as well as by classicla means. We found that two of the impurities responsible for the chromatographic behaviour of acetonitrile are acetamisde and ethyleneimine or its oligomers.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
