Abstract
Changes in soluble proteins of the maturing cataract were studied in mice of the mutant stocks: ocular retardation (gene symbol or ); white ( Mi wh ); fidget ( fi ); and dominant cataract-Fraser ( Cat Fr ). Crystallin fractions of the normal lens were first identified by polyacrylamide gel electrophoresis. In the course of cataract development in mice of the studied mutant stocks, a diminution or loss of some crystallin fractions was observed. The maturation of secondary cataracts in or/or, Mi wh / Mi wh , and fi/fi mice was accompanied by a significant increase in serum albumin, which preceded visible lens opacity. In agar electrophoresis, albumin and α-crystallin were found to migrate together, simulating quantitative maintenance of α-crystallin in the maturing cataract. Another serum protein, electrophoretically similar to transferrin, also penetrated into the lens during cataract development. Maturation of primary nuclear cataract in Cat Fr / Cat Fr mice was not accompanied by increased penetration of serum proteins into the lens. Apparently, penetration of serum proteins into the lens participates in the pathogenesis of secondary subcapsular cataracts, while primary nuclear cataracts have a different etiology.
Published Version
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