Abstract
AbstractCell‐mediated immune response to tumour‐associated antigens (taa) extracted from syngeneic methylcholanthrene (mc)‐induced mouse sarcomas with 3 m kci was simultaneously examined by in vitro sensitization of lymph‐node cells (lnc), macrophage electrophoretic mobility (mem) assay and transplantation tests. the solubilized taa elicited in vivo both primary and secondary immune responses as judged from induction of transplantation resistance. the resistance to transplantation of the corresponding sarcoma cells was tumour‐specific and relative in nature, i.e., only partial and weaker than that induced by immunization with irradiated tumour tissue. treatment of mice with solubilized taa prior to immunization with irradiated tumour tissue inhibited the induction of transplantation resistance by irradiated tumour cells. the interaction of the solubilized taa preparations in vitro, either with lnc from animals immunized against the corresponding tumour or with lnc from tumour‐bearing animals, gave rise to a macrophage‐slowing factor detectable by the mem test. taa detected by the mem assay in kci extracts of individual mc‐induced sarcomas were found to be non‐cross‐reacting, individually distinct, like those detected by transplantation tests. individual specificity of taa present in the mc‐induced sarcomas was also demonstrated in vitro by sensitization of normal syngeneic lnc on monolayers of irradiated sarcoma cells followed by detection of the generated cytotoxic lnc in a cytotoxicity assay utilizing the inhibition of [3h]thymidine incorporation. whereas sensitization of lnc on layers of irradiated sarcoma cells gave rise to lymphocytes cytotoxic for cells of the sensitizing tumour, sensitization of lnc with solubilized taa isolated from the same sarcoma has thus far given negative results.
Published Version
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