SOLID-STATE 13C NMR SPECTROSCOPY IN POLYSACCHARIDE ANALYSIS

Abstract

Polysaccharides are high molecular weight compounds represented by long linear and/or branched chains of monosaccharide residues linked by a glycosidic bond. Currently, there is a huge and rapidly-growing interest in the chemistry of polysaccharides due to their widespread use in various spheres of human life. The study of polysaccharide structure is a complex and non-trivial task, and in this area solid-state 13C NMR spectroscopy are widely applied in recent years. The review analyzes the possibilities of solid-state 13C NMR spectroscopy for the study of polysaccharides and natural objects containing polysaccharides. The evolution of 13C solid-state NMR spectroscopy methods is shown with the main focus on the usage of the cross-polarization (CP) technique based on rotating the sample under the magic angle (MAS), since in this case the spectra are obtained without artifacts signals and with the best signal-to-noise ratio and high resolution. The review focuses on cellulose as the most widespread polysaccharide, in addition, the applicability of CP-MAS 13C NMR spectroscopy for the study of other polysaccharides, as well as plant materials, is considered. The represented examples clearly show that CP-MAS 13C NMR spectroscopy is the most powerful experimental method that allows to obtain information on both the composition and structure of polysaccharides, as well as the composition of various plant materials. Moreover, the combination of available equipment and various techniques of solid-state 13C NMR experiment will contribute to the progress of further research in the chemistry of polysaccharides and their derivatives.