Abstract
The use of solid supported membranes (SSM) was investigated for reconstitution of ion channels and for potential application to screen pharmacological reagents affecting ion channel function. The voltage-gated Kv1.5 K + channel was reconstituted on an SSM and a current was measured. This current was dependent on the presence of K +, but not Na +, indicating that the Kv1.5 K + channel maintained cation specificity when reconstituted on SSM. Two pharmacological reagents applied to Kv1.5 K + channels reconstituted on SSM had similar inhibitory effects as those measured using Kv1.5 in biological membranes. SSM-mounted ion channels were stable enough to be washed with buffer solution and reused many times, allowing solution exchange essential for pharmacological drug screening.
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