Solid core liposomes with encapsulated colloidal gold particles

Abstract

Solid core liposomes with encapsulated colloidal gold particles were prepared through four major steps: (1) Preparation of prevesicles with encapsulated solid cores of agarose-gelatin by emulsification of agarose-gelatin sol in organic solvent containing emulsifiers followed by cooling. (2) Extraction of lipophilic components from prevesicles to obtain microspherules of agarose-gelatin. (3) Introducing colloidal gold particles into microspherules and coating with protein molecules. (4) Encapsulation of colloidal gold-bearing microspherules with the modified organic solvent spherule evaporation method for preparation of liposomes (Kim et al. (1983) Biochim. Biophys. Acta 728, 339–348 and Kim et al. (1984) Biochim. Biophys. Acta 812, 793–801). Electron micrographs showed that if liposomes were prepared by using a lipid mixture containing dioleoylphosphatidylcholine/cholesterol/dioleoylphosphatidylglycerol/triolein (molar ratio 4.5:4.5:1:1), there was only a single continuous bilayer membrane for each solid core liposome. However, if no triolein was added to the lipid mixture, it would cause the formation of multilamellar liposomes. In both cases, there were hundreds to thousands of colloidal gold particles within each solid core liposome.